Journal: Oncology reports

Article Title: Human ether-à-go-go-related gene expression is essential for cisplatin to induce apoptosis in human gastric cancer.

doi: 10.3892/or.2011.1515

Figure Lengend Snippet: Figure 1. SGC7901 and MGC803 cells were incubated with HERG siRNA (A) or different concentrations of cisplatin (C) for 48 h, and cells were lysed and subjected to Western blot analysis to detect expression of HERG. (B and D) The density of each band (from A and C, respectively) was measured and compared with that of the internal control, GAPDH. *Significant difference in band density from the control.

Article Snippet: The control siRNA and HERG siRNA were purchased from Santa Cruz Biotechnology.

Techniques: Incubation, Western Blot, Expressing, Control

Journal: Oncology reports

Article Title: Human ether-à-go-go-related gene expression is essential for cisplatin to induce apoptosis in human gastric cancer.

doi: 10.3892/or.2011.1515

Figure Lengend Snippet: Figure 2. SGC7901 (A) and MGC803 (B) cells transfected with control siRNA or HERG siRNA were incubated with different concentrations of cisplatin. Forty-eight hours later, the proliferation of cells was assessed by the CCK8 method to calculate the proliferation inhibition rate. The dotted lines show the concentration of cisplatin, which resulted in 50% of maximal proliferation inhibition (IC50) of cells.

Article Snippet: The control siRNA and HERG siRNA were purchased from Santa Cruz Biotechnology.

Techniques: Transfection, Control, Incubation, Inhibition, Concentration Assay

Journal: Oncology reports

Article Title: Human ether-à-go-go-related gene expression is essential for cisplatin to induce apoptosis in human gastric cancer.

doi: 10.3892/or.2011.1515

Figure Lengend Snippet: Figure 3. SGC7901 (A) and MGC803 (B) cells transfected with control siRNA or HERG siRNA were incubated with or without cisplatin. Forty-eight hours later, cells were harvested and flow cytometry was performed to measure apoptosis rates. *Significant increase in the apoptosis rate from control; #Significant increase compared with cisplatin-treated cells. (C) Representative histograms from cytometrically analyzed SGC7901 (upper panel) and MGC803 (lower panel) cells treated with control siRNA, HERG siRNA, cisplatin + control siRNA or cisplatin + HERG siRNA. (D) Cells were viewed by laser scanning confocal microscopy. Representative photographs were taken of SGC7901 (upper panel) and MGC803 (lower panel) cells treated with control siRNA, HERG siRNA, cisplatin + control siRNA or cisplatin + HERG siRNA.

Article Snippet: The control siRNA and HERG siRNA were purchased from Santa Cruz Biotechnology.

Techniques: Transfection, Control, Incubation, Flow Cytometry, Confocal Microscopy

Journal: Oncology reports

Article Title: Human ether-à-go-go-related gene expression is essential for cisplatin to induce apoptosis in human gastric cancer.

doi: 10.3892/or.2011.1515

Figure Lengend Snippet: Figure 4. (A) SGC7901 and MGC803 cells were treated with control siRNA (lane 1), cisplatin + control siRNA (lane 2), or cisplatin + HERG siRNA (lane 3) for 48 h. Homogenates of the cells were subjected to Western blot analysis to detect the expression of HERG, Bcl-2, Bax and active caspase-3. (B) The density of each band was measured and compared with that of the internal control, GAPDH. *Significant difference in band density from control siRNA group. #Significant difference from cisplatin + control siRNA group.

Article Snippet: The control siRNA and HERG siRNA were purchased from Santa Cruz Biotechnology.

Techniques: Control, Western Blot, Expressing

Journal: Oncology reports

Article Title: Human ether-à-go-go-related gene expression is essential for cisplatin to induce apoptosis in human gastric cancer.

doi: 10.3892/or.2011.1515

Figure Lengend Snippet: Figure 5. Mice were subcutaneously injected with SGC7901 cells. When tumors reached around 100 mm3 in volume, mice received treatment with control siRNA, HERG siRNA, cisplatin + control siRNA, or cisplatin + HERG siRNA. (A) HERG expression in subcutaneous SGC7901 tumors was detected by immu nohistochemical analyses. Sections were prepared from tumors 1 week after receiving treatment. (B) Tumoral cell apoptosis was detected by TUNEL. Illustrated are representative sections prepared from subcutaneous tumors 2 weeks after treatment. (C) TUNEL-positive cells were counted to give the apoptosis index. (D) Two weeks after treatment, tumors from control mice (lane 1), cisplatin + control siRNA (lane 2), and cisplatin + HERG siRNA (lane 3) were homogenized and subjected to Western blot analysis to measure the expression of HERG, Bcl-2, Bax and active caspase-3. (E) The density of each band from (D) was measured and compared to that of the internal control, GAPDH. (F) The sizes (mm3) of tumors were monitored and recorded for 3 weeks. *Significant difference from control siRNA group.

Article Snippet: The control siRNA and HERG siRNA were purchased from Santa Cruz Biotechnology.

Techniques: Injection, Control, Expressing, TUNEL Assay, Western Blot